Charles D. Hufford
Associate Dean for Research and Graduate Programs
Professor of Pharmacognosy
Research Professor in the Research Institute of Pharmaceutical Sciences
Dr. Hufford received his B.S in Pharmacy (1967) and Ph.D in Pharmacognosy and Natural Products Chemistry (1972) from The Ohio State University. He joined the faculty of The University of Mississippi in 1972 as an Assistant Professor of Pharmacognosy. He was promoted to Associate Professor in 1976 and Professor in 1982. He served as Acting Chair of the Pharmacognosy Department (1977-78, 1984-85) and was appointed Chair in 1987. He was appointed to his current position as Associate Dean in 1995 and continued to serve as acting chair, until 1999.
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Research in Progress
Dr. Hufford has concentrated much of his effort on antibiotic discovery through the development of an antimicrobial screening program that is representative of common human pathogens including bacteria and fungi. Dr. Hufford has focused a major effort on the evaluation of antibiotic activity from higher plants. More recently, he has concentrated on the discovery of prototype antifungal antibiotics effective against opportunistic infections commonly found in the AIDS patient. Significant efforts are being devoted to the discovery of new agents for systemic Candida and Cryptococcus infections. This is accomplished by using bioassay directed fractionation procedures upon active plant extracts. The active compounds are then subjected to structure elucidation methods utilizing modern spectroscopic techniques, especially 2D-NMR methods.
Another major research interest includes the utilization of fungi as predictors of mammalian drug metabolites. This system has been applied successfully to antimalarial drugs (primaquine and artemisinin/arteether) and several others as well. The use of the fungal model system as an in vitro predictor for mammalian drug metabolites overcomes most if not all of the obstacles encountered when utilizing traditional in vitro or in vivo mammalian model systems. The major advantage is the ability of microorganisms to produce significant quantities of metabolites that would be difficult to obtain from either animal systems or chemical synthesis. Other advantages include the greatly reduced cost of carrying out microbial metabolism studies as compared to animal metabolism studies. Furthermore, microbial metabolic transformations parallel very closely with human biotransformations, while in many cases, the whole animal and in vitro mammalian model systems differ from human metabolism due to significant species variations. In addition, the total number of research animals necessary for the evaluation of the metabolic profile of a drug can be significantly reduced when microorganisms are used as predictive models for initial studies.
In addition to utilizing microorganisms as models for predicting mammalian drug metabolites, Dr. Hufford has also conducted studies to utilize microorganisms to effect specific changes in structures of biologically active substances. It is well known that microorganisms can carry out regiospecific and stereospecific transformations of complex molecules often in a high yielding manner. Dr. Hufford seeks to utilize microbial systems to transform biologically active substances that may not be amenable to chemical transformations. Examples of projects in this area include the microbial modification of the diterpene stemodin, which closely resembles the antiviral aphidicolin, and the transformation of several biologically active sesquiterpenes.
His interests in herbal remedies dates back to his early years in Pharmacognosy at Ohio State and continues to this day. He currently teaches information about herbal remedies to undergraduate pharmacy students.
S. Liu, B. Oguntimein, C.D. Hufford and A.M. Clark, "3-Methoxysampangine, A Novel Antifungal Copyrine Alkaloid from Cleistopholis patens", Antimicrob. Ag. Chemother., 34, 529-533 (1990).
C.D. Hufford, B.O. Oguntimein, and I. Muhammad, "New Stemodane Diterpenes from Stemodia maritima", J. Nat. Prod., 55, 48-52 (1992).
C.D. Hufford, Y.Jia, E.M. Croom, I. Muhammed, A.K. Okunade and A.M. Clark, "Antimicrobial Compounds from Petalostemum Purpureum", J. Nat. Prod., 56, 1878-1889 (1993).
S.I. Khalifa, J.K. Baker, R.D. Rogers, F.S. El-Feraly and C.D. Hufford, "The Microbial and Mammalian Metabolism Studies of the Semisynthetic Antimalarial, Anhydrodihydroartemisinin", Pharm. Res., 11, 990-994 (1994).
E. Li, A.M. Clark and C.D. Hufford, "Antifungal Evaluation of Pseudolaric Acid B, A Major Constituent of Pseudolarix Kaempferi", J. Nat. Prod., 58, 57-67 (1995).
C.D. Hufford, S.I. Khalifa, K.Y. Orabi, F.T. Wiggers, R. Kumar, R.D. Rogers and C.F. Campana, "C-1 Hydroxyarteether, A New Microbial Transformation Product", J. Nat. Prod., 58, 751-755 (1995).
E.A. Abourashed, A.M. Clark and C.D. Hufford, "Microbial Models of Mammalian Metabolism of Xenobiotics: An Updated Review", Current Medicinal Chemistry, 6, 359-374 (1999).
E.A. Abourashed, F.S. El-Feraly and C.D. Hufford, "Carboxylic Acid Microbial Metabolites of the Natural Benzoquinone, Maesanin", J. Nat. Prod., 62, 714-716 (1999).
K.Y. Orabi, E. Li, A.M. Clark and C.D. Hufford, "Microbial Transformation of Sampangine", J. Nat. Prod., 62, 988-992 (1999).
X.-C. Li, H.N. ElSohly, C.D. Hufford and A.M. Clark – “NMR assignments of ellagic acid derivatives”, Magnetic Resonance in Chemistry, 37, pp. 856-859 (1999).
E.A. Abourashed, F.S. ElFeraly, I.A. Khan and C.D. Hufford - "2-Hydroxy-5- (ethnolamino)-3-(10'-Z-pentadecenyl)-1,4-benzoquinone, NewMicrobial Phase II Metabolite of Maesanin", Chem. Pharm. Bull., 48, pp. 45-47 (2000).
K.Y. Orabi, A.M. Clark and C.D. Hufford, "Microbial Transformation of Benzosampangine"", J. Nat. Prod., 63, 396-398 (2000).
K.Y. Orabi, L.A. Walker, A.M. Clark and C.D. Hufford – “Characterization of the Major Metabolite of Sampangine in Rats”, J. Nat. Prod., 63, pp. 685-687 (2000).
I. Muhammad, K.A. ElSayed, J.S. Mossa, M.S. AlSaid, F.S. El-Feraly, A.M. Clark, C.D. Hufford, S. Oh and A.M.S. Mayer – “Bioactive 12-Oleanene Triterpene and Secotriterpene Acids from Maytenus undata”, J. Nat. Prod., 63, pp. 605-610 (2000).
E.A. Abourashed, I. Muhammad, M. Resch, R. Bauer, F.S. El-Feraly and C.D. Hufford – “Inhibitory Effects of Maesanin and Analogs on Arachidonic Acid Metabolizing Enzymes”, Planta Med., 67, pp. 360-361 (2001).
A.J. Al-Rehaily, T.A. Al-Howiriny, M.S. Ahmad, M.A. Al-Yahya, F.S. El-Feraly, C.D. Hufford and A.T. McPhail – “Alkaloids from Haplophyllum tuberculatum”, Phytochemistry, 57, pp. 597-602 (2001).
S.Y. Ablordeppey, P. Fan, S. Li, A.M. Clark and C.D. Hufford – “Substituted Indoloquinolines as New Antifungal Agents”, Bioorganic & Medicinal Chemistry, 10, pp. 1337-1346 (2002).
W.G. Chambliss, C.D. Hufford, M.L. Flagg and J.K. Glisson – “Assessment of the Quality of Reference Books on Botanical Dietary Supplements”, Journal of the American Pharmaceutical Association, 42, pp. 723-734 (2002).
J.G. Topliss, A.M. Clark, E. Ernst, C.D. Hufford, G.A.R. Johnston, J.M. Rimoldi and B.J. Weimann – “Natural and Synthetic Substances Related to Human Health (IUPAC Technical Report)”, Pure and Applied Chemistry, 74, pp. 1957-1985 (2002).
J.K. Glisson, H.E. Rogers, E.A. Abourashed, R. Ogletree, C.D. Hufford and I.A. Khan - "Clinic at the Health Food Store? Employee Recommendations and Product Analysis", Pharmacotherapy, 23, pp. 64-72 (2003).
V. Samoylenko, S.I. Khan, M.R. Jacob, B.L. Tekwani, L.A. Walker, C.D. Hufford, and I. Muhammad, "Bioactive (+)-Manzamine A and (+)-8-Hydroxymanzamaine A Tertiary Bases and Salts from Acanthostrongylophora ingens and their Preparations", Natural Product Communications, 4, (2009)